Construct: shRNA TRCN0000047421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371755.2-750s1c1
DNA Barcode:
CCAACAAATTTGTGCGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC401198 (401198)
Gene Description:
similar to Rho-guanine nucleotide exchange factor (Rho-interacting protein 2) (RhoGEF) (RIP2)
Transcript:
RefSeq XM_371755.2 (NON-CURRENT)
Match location:
Position 750 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAAATTTGTGCGTATTT
Hairpin Sequence:
5'-CCGG-CCAACAAATTTGTGCGTATTT-CTCGAG-AAATACGCACAAATTTGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAAATTTGTGCGTATTTCTCGAGAAATACGCACAAATTTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAAATTTGTGCGTATTTCTCGAGAAATACGCACAAATTTGTTGG-3'

Other clones with same target sequence:

(none)