Construct: shRNA TRCN0000047434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014470.2-741s1c1
DNA Barcode:
GCTCTGAACTCATCTCTTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RND1 (27289)
Gene Description:
Rho family GTPase 1
Transcript:
RefSeq NM_014470.2 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGAACTCATCTCTTCTA
Hairpin Sequence:
5'-CCGG-GCTCTGAACTCATCTCTTCTA-CTCGAG-TAGAAGAGATGAGTTCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGAACTCATCTCTTCTACTCGAGTAGAAGAGATGAGTTCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGAACTCATCTCTTCTACTCGAGTAGAAGAGATGAGTTCAGAGC-3'

Other clones with same target sequence:

(none)