Construct: shRNA TRCN0000047463

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014784.2-5941s1c1
DNA Barcode:
CCCTCAGACATGCAAGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF11 (9826)
Gene Description:
Rho guanine nucleotide exchange factor 11
Transcript:
RefSeq NM_014784.2 (NON-CURRENT)
Match location:
Position 5941 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047463)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGACATGCAAGTGAAA
Hairpin Sequence:
5'-CCGG-CCCTCAGACATGCAAGTGAAA-CTCGAG-TTTCACTTGCATGTCTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGACATGCAAGTGAAACTCGAGTTTCACTTGCATGTCTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGACATGCAAGTGAAACTCGAGTTTCACTTGCATGTCTGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000298933