Construct: shRNA TRCN0000047507

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014448.2-979s1c1
DNA Barcode:
GCTGGACTTCAGCAAGGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF16 (27237)
Gene Description:
Rho guanine nucleotide exchange factor 16
Transcript:
RefSeq NM_014448.2 (NON-CURRENT)
Match location:
Position 979 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047507)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGACTTCAGCAAGGTCAA
Hairpin Sequence:
5'-CCGG-GCTGGACTTCAGCAAGGTCAA-CTCGAG-TTGACCTTGCTGAAGTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGACTTCAGCAAGGTCAACTCGAGTTGACCTTGCTGAAGTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGACTTCAGCAAGGTCAACTCGAGTTGACCTTGCTGAAGTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000289517