Construct: shRNA TRCN0000047524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020345.2-547s1c1
DNA Barcode:
GTGAATAACCTTGAATCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NKIRAS1 (28512)
Gene Description:
NFKB inhibitor interacting Ras like 1
Transcript:
RefSeq NM_020345.2 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAATAACCTTGAATCCTTT
Hairpin Sequence:
5'-CCGG-GTGAATAACCTTGAATCCTTT-CTCGAG-AAAGGATTCAAGGTTATTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAATAACCTTGAATCCTTTCTCGAGAAAGGATTCAAGGTTATTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAATAACCTTGAATCCTTTCTCGAGAAAGGATTCAAGGTTATTCAC-3'

Other clones with same target sequence:

(none)