Construct: shRNA TRCN0000047536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015318.2-2065s1c1
DNA Barcode:
GAATGGCAGTTTCAAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF18 (23370)
Gene Description:
Rho/Rac guanine nucleotide exchange factor 18
Transcript:
RefSeq NM_015318.2 (NON-CURRENT)
Match location:
Position 2065 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGCAGTTTCAAGAAGAA
Hairpin Sequence:
5'-CCGG-GAATGGCAGTTTCAAGAAGAA-CTCGAG-TTCTTCTTGAAACTGCCATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGCAGTTTCAAGAAGAACTCGAGTTCTTCTTGAAACTGCCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGCAGTTTCAAGAAGAACTCGAGTTCTTCTTGAAACTGCCATTC-3'

Other clones with same target sequence:

  1. TRCN0000289759