Construct: shRNA TRCN0000047565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014850.1-1201s1c1
DNA Barcode:
GCCACCCTCAAGATAGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRGAP3 (9901)
Gene Description:
SLIT-ROBO Rho GTPase activating protein 3
Transcript:
RefSeq NM_014850.1 (NON-CURRENT)
Match location:
Position 1201 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCCTCAAGATAGAGAAT
Hairpin Sequence:
5'-CCGG-GCCACCCTCAAGATAGAGAAT-CTCGAG-ATTCTCTATCTTGAGGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCCTCAAGATAGAGAATCTCGAGATTCTCTATCTTGAGGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCCTCAAGATAGAGAATCTCGAGATTCTCTATCTTGAGGGTGGC-3'

Other clones with same target sequence:

(none)