Construct: shRNA TRCN0000047582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016339.1-1270s1c1
DNA Barcode:
GAAGGGAGTAAGACCCTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAPGEFL1 (51195)
Gene Description:
Rap guanine nucleotide exchange factor like 1
Transcript:
RefSeq NM_016339.1 (NON-CURRENT)
Match location:
Position 1270 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGGAGTAAGACCCTTGTA
Hairpin Sequence:
5'-CCGG-GAAGGGAGTAAGACCCTTGTA-CTCGAG-TACAAGGGTCTTACTCCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGGAGTAAGACCCTTGTACTCGAGTACAAGGGTCTTACTCCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGGAGTAAGACCCTTGTACTCGAGTACAAGGGTCTTACTCCCTTC-3'

Other clones with same target sequence:

(none)