Construct: shRNA TRCN0000047584

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_208859.3-946s1c1
DNA Barcode:
CGATGGTGGAACCAGATAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC283816 (283816)
Gene Description:
similar to RIKEN cDNA 1810036I24
Transcript:
RefSeq XM_208859.3 (NON-CURRENT)
Match location:
Position 946 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047584)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGGTGGAACCAGATAGAT
Hairpin Sequence:
5'-CCGG-CGATGGTGGAACCAGATAGAT-CTCGAG-ATCTATCTGGTTCCACCATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGGTGGAACCAGATAGATCTCGAGATCTATCTGGTTCCACCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGGTGGAACCAGATAGATCTCGAGATCTATCTGGTTCCACCATCG-3'

Other clones with same target sequence:

(none)