Construct: shRNA TRCN0000047639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291085.1-3906s1c1
DNA Barcode:
CCTCATTTAGTTCACCTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP31 (57514)
Gene Description:
Rho GTPase activating protein 31
Transcript:
RefSeq XM_291085.1 (NON-CURRENT)
Match location:
Position 3906 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTTAGTTCACCTGGAA
Hairpin Sequence:
5'-CCGG-CCTCATTTAGTTCACCTGGAA-CTCGAG-TTCCAGGTGAACTAAATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTTAGTTCACCTGGAACTCGAGTTCCAGGTGAACTAAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTTAGTTCACCTGGAACTCGAGTTCCAGGTGAACTAAATGAGG-3'

Other clones with same target sequence:

(none)