Construct: shRNA TRCN0000047642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291085.1-2825s1c1
DNA Barcode:
CCACAAGCAACAGTGGAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP31 (57514)
Gene Description:
Rho GTPase activating protein 31
Transcript:
RefSeq XM_291085.1 (NON-CURRENT)
Match location:
Position 2825 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAAGCAACAGTGGAAGTA
Hairpin Sequence:
5'-CCGG-CCACAAGCAACAGTGGAAGTA-CTCGAG-TACTTCCACTGTTGCTTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAAGCAACAGTGGAAGTACTCGAGTACTTCCACTGTTGCTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAAGCAACAGTGGAAGTACTCGAGTACTTCCACTGTTGCTTGTGG-3'

Other clones with same target sequence:

(none)