Construct: shRNA TRCN0000047674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001659.1-663s1c1
DNA Barcode:
GATGCTGTACTCCTTGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARF3 (377)
Gene Description:
ADP ribosylation factor 3
Transcript:
RefSeq NM_001659.1 (NON-CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCTGTACTCCTTGTCTTT
Hairpin Sequence:
5'-CCGG-GATGCTGTACTCCTTGTCTTT-CTCGAG-AAAGACAAGGAGTACAGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCTGTACTCCTTGTCTTTCTCGAGAAAGACAAGGAGTACAGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCTGTACTCCTTGTCTTTCTCGAGAAAGACAAGGAGTACAGCATC-3'

Other clones with same target sequence:

(none)