Construct: shRNA TRCN0000047684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018098.4-441s1c1
DNA Barcode:
GCCCGTTGTATTGTACAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ECT2 (1894)
Gene Description:
epithelial cell transforming 2
Transcript:
RefSeq NM_018098.4 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGTTGTATTGTACAAGTA
Hairpin Sequence:
5'-CCGG-GCCCGTTGTATTGTACAAGTA-CTCGAG-TACTTGTACAATACAACGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGTTGTATTGTACAAGTACTCGAGTACTTGTACAATACAACGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGTTGTATTGTACAAGTACTCGAGTACTTGTACAATACAACGGGC-3'

Other clones with same target sequence:

  1. TRCN0000299290