Construct: shRNA TRCN0000047805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020919.2-3984s1c1
DNA Barcode:
CCTACTTCAAACCTAGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALS2 (57679)
Gene Description:
alsin Rho guanine nucleotide exchange factor ALS2
Transcript:
RefSeq NM_020919.2 (NON-CURRENT)
Match location:
Position 3984 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACTTCAAACCTAGTCTAT
Hairpin Sequence:
5'-CCGG-CCTACTTCAAACCTAGTCTAT-CTCGAG-ATAGACTAGGTTTGAAGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACTTCAAACCTAGTCTATCTCGAGATAGACTAGGTTTGAAGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACTTCAAACCTAGTCTATCTCGAGATAGACTAGGTTTGAAGTAGG-3'

Other clones with same target sequence:

(none)