Construct: shRNA TRCN0000047830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014488.3-571s1c1
DNA Barcode:
CTGCGGGAGATAGAACAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB30 (27314)
Gene Description:
RAB30, member RAS oncogene family
Transcript:
RefSeq NM_014488.3 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCGGGAGATAGAACAATAT
Hairpin Sequence:
5'-CCGG-CTGCGGGAGATAGAACAATAT-CTCGAG-ATATTGTTCTATCTCCCGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCGGGAGATAGAACAATATCTCGAGATATTGTTCTATCTCCCGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCGGGAGATAGAACAATATCTCGAGATATTGTTCTATCTCCCGCAG-3'

Other clones with same target sequence:

(none)