Construct: shRNA TRCN0000047920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006788.3-1581s1c1
DNA Barcode:
GCACAAGAGATAGCCAGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALBP1 (10928)
Gene Description:
ralA binding protein 1
Transcript:
RefSeq NM_006788.3 (NON-CURRENT)
Match location:
Position 1581 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGAGATAGCCAGTCTT
Hairpin Sequence:
5'-CCGG-GCACAAGAGATAGCCAGTCTT-CTCGAG-AAGACTGGCTATCTCTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGAGATAGCCAGTCTTCTCGAGAAGACTGGCTATCTCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGAGATAGCCAGTCTTCTCGAGAAGACTGGCTATCTCTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000291459