Construct: shRNA TRCN0000047967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015178.1-2098s1c1
DNA Barcode:
GCACCAACTACAACAACGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RHOBTB2 (23221)
Gene Description:
Rho related BTB domain containing 2
Transcript:
RefSeq NM_015178.1 (NON-CURRENT)
Match location:
Position 2098 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000047967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCAACTACAACAACGTGT
Hairpin Sequence:
5'-CCGG-GCACCAACTACAACAACGTGT-CTCGAG-ACACGTTGTTGTAGTTGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCAACTACAACAACGTGTCTCGAGACACGTTGTTGTAGTTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCAACTACAACAACGTGTCTCGAGACACGTTGTTGTAGTTGGTGC-3'

Other clones with same target sequence:

(none)