Construct: shRNA TRCN0000048076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016370.1-849s1c1
DNA Barcode:
TGACTTGAACAGTGGCTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB9B (51209)
Gene Description:
RAB9B, member RAS oncogene family
Transcript:
RefSeq NM_016370.1 (NON-CURRENT)
Match location:
Position 849 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACTTGAACAGTGGCTCCAA
Hairpin Sequence:
5'-CCGG-TGACTTGAACAGTGGCTCCAA-CTCGAG-TTGGAGCCACTGTTCAAGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACTTGAACAGTGGCTCCAACTCGAGTTGGAGCCACTGTTCAAGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACTTGAACAGTGGCTCCAACTCGAGTTGGAGCCACTGTTCAAGTCA-3'

Other clones with same target sequence:

(none)