Construct: shRNA TRCN0000048157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006939.1-3828s1c1
DNA Barcode:
GCTCAGTTCTAGTCAGAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOS2 (6655)
Gene Description:
SOS Ras/Rho guanine nucleotide exchange factor 2
Transcript:
RefSeq NM_006939.1 (NON-CURRENT)
Match location:
Position 3828 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGTTCTAGTCAGAATAA
Hairpin Sequence:
5'-CCGG-GCTCAGTTCTAGTCAGAATAA-CTCGAG-TTATTCTGACTAGAACTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGTTCTAGTCAGAATAACTCGAGTTATTCTGACTAGAACTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGTTCTAGTCAGAATAACTCGAGTTATTCTGACTAGAACTGAGC-3'

Other clones with same target sequence:

(none)