Construct: shRNA TRCN0000048199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024979.2-658s1c1
DNA Barcode:
CGTACCAGACTTACACGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCF2L (23263)
Gene Description:
MCF.2 cell line derived transforming sequence like
Transcript:
RefSeq NM_024979.2 (NON-CURRENT)
Match location:
Position 658 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACCAGACTTACACGGTTA
Hairpin Sequence:
5'-CCGG-CGTACCAGACTTACACGGTTA-CTCGAG-TAACCGTGTAAGTCTGGTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACCAGACTTACACGGTTACTCGAGTAACCGTGTAAGTCTGGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACCAGACTTACACGGTTACTCGAGTAACCGTGTAAGTCTGGTACG-3'

Other clones with same target sequence:

(none)