Construct: shRNA TRCN0000048282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006421.2-5467s1c1
DNA Barcode:
GCCTGGACTAACTTACTGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARFGEF1 (10565)
Gene Description:
ADP ribosylation factor guanine nucleotide exchange factor 1
Transcript:
RefSeq NM_006421.2 (NON-CURRENT)
Match location:
Position 5467 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGACTAACTTACTGCTT
Hairpin Sequence:
5'-CCGG-GCCTGGACTAACTTACTGCTT-CTCGAG-AAGCAGTAAGTTAGTCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGACTAACTTACTGCTTCTCGAGAAGCAGTAAGTTAGTCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGACTAACTTACTGCTTCTCGAGAAGCAGTAAGTTAGTCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000289950