Construct: shRNA TRCN0000048324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_084445.9-324s1c1
DNA Barcode:
CCATGTATCTACTGTGCGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC143158 (143158)
Gene Description:
similar to centaurin, gamma-like family, member 1; ARF GTPase-activating protein; Em:AC012044.1
Transcript:
RefSeq XM_084445.9 (NON-CURRENT)
Match location:
Position 324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTATCTACTGTGCGTTT
Hairpin Sequence:
5'-CCGG-CCATGTATCTACTGTGCGTTT-CTCGAG-AAACGCACAGTAGATACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTATCTACTGTGCGTTTCTCGAGAAACGCACAGTAGATACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTATCTACTGTGCGTTTCTCGAGAAACGCACAGTAGATACATGG-3'

Other clones with same target sequence:

  1. TRCN0000153993