Construct: shRNA TRCN0000048348

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004703.2-374s1c1
DNA Barcode:
GCAGTATTACAAGCTGCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RABEP1 (9135)
Gene Description:
rabaptin, RAB GTPase binding effector protein 1
Transcript:
RefSeq NM_004703.2 (NON-CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048348)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTATTACAAGCTGCACAA
Hairpin Sequence:
5'-CCGG-GCAGTATTACAAGCTGCACAA-CTCGAG-TTGTGCAGCTTGTAATACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTATTACAAGCTGCACAACTCGAGTTGTGCAGCTTGTAATACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTATTACAAGCTGCACAACTCGAGTTGTGCAGCTTGTAATACTGC-3'

Other clones with same target sequence:

(none)