Construct: shRNA TRCN0000048367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_208443.4-339s1c1
DNA Barcode:
CCTGGTCTACGACATTGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAR2 (286526)
Gene Description:
Ras-like GTPase-like
Transcript:
RefSeq XM_208443.4 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGTCTACGACATTGCAAA
Hairpin Sequence:
5'-CCGG-CCTGGTCTACGACATTGCAAA-CTCGAG-TTTGCAATGTCGTAGACCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGTCTACGACATTGCAAACTCGAGTTTGCAATGTCGTAGACCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGTCTACGACATTGCAAACTCGAGTTTGCAATGTCGTAGACCAGG-3'

Other clones with same target sequence:

(none)