Construct: shRNA TRCN0000048395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495815.1-308s1c1
DNA Barcode:
CGAGGTTCCAAAGCCCAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC439967 (439967)
Gene Description:
similar to centaurin, gamma-like family, member 1; ARF GTPase-activating protein; Em:AC012044.1
Transcript:
RefSeq XM_495815.1 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGTTCCAAAGCCCAAATA
Hairpin Sequence:
5'-CCGG-CGAGGTTCCAAAGCCCAAATA-CTCGAG-TATTTGGGCTTTGGAACCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGTTCCAAAGCCCAAATACTCGAGTATTTGGGCTTTGGAACCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGTTCCAAAGCCCAAATACTCGAGTATTTGGGCTTTGGAACCTCG-3'

Other clones with same target sequence:

(none)