Construct: shRNA TRCN0000048397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495815.1-907s1c1
DNA Barcode:
CTCACGTCAAAGGTCTGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC439967 (439967)
Gene Description:
similar to centaurin, gamma-like family, member 1; ARF GTPase-activating protein; Em:AC012044.1
Transcript:
RefSeq XM_495815.1 (NON-CURRENT)
Match location:
Position 907 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACGTCAAAGGTCTGTCTT
Hairpin Sequence:
5'-CCGG-CTCACGTCAAAGGTCTGTCTT-CTCGAG-AAGACAGACCTTTGACGTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACGTCAAAGGTCTGTCTTCTCGAGAAGACAGACCTTTGACGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACGTCAAAGGTCTGTCTTCTCGAGAAGACAGACCTTTGACGTGAG-3'

Other clones with same target sequence:

(none)