Construct: shRNA TRCN0000048399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495823.1-1569s1c1
DNA Barcode:
CCGTTCCAAATATGAGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTGLF4 (439975)
Gene Description:
centaurin, gamma-like family, member 4
Transcript:
RefSeq XM_495823.1 (NON-CURRENT)
Match location:
Position 1569 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTCCAAATATGAGGAGAA
Hairpin Sequence:
5'-CCGG-CCGTTCCAAATATGAGGAGAA-CTCGAG-TTCTCCTCATATTTGGAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTCCAAATATGAGGAGAACTCGAGTTCTCCTCATATTTGGAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTCCAAATATGAGGAGAACTCGAGTTCTCCTCATATTTGGAACGG-3'

Other clones with same target sequence:

  1. TRCN0000154162