Construct: shRNA TRCN0000048403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495830.1-5394s1c1
DNA Barcode:
AGGAAATCACAAATTCAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTGLF2 (439983)
Gene Description:
centaurin, gamma-like family, member 2
Transcript:
RefSeq XM_495830.1 (NON-CURRENT)
Match location:
Position 5394 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAAATCACAAATTCAGCTA
Hairpin Sequence:
5'-CCGG-AGGAAATCACAAATTCAGCTA-CTCGAG-TAGCTGAATTTGTGATTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAAATCACAAATTCAGCTACTCGAGTAGCTGAATTTGTGATTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAAATCACAAATTCAGCTACTCGAGTAGCTGAATTTGTGATTTCCT-3'

Other clones with same target sequence:

(none)