Construct: shRNA TRCN0000048470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290799.4-871s1c1
DNA Barcode:
GCTCTGATCCAGAATAGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP23 (57636)
Gene Description:
Rho GTPase activating protein 23
Transcript:
RefSeq XM_290799.4 (NON-CURRENT)
Match location:
Position 871 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGATCCAGAATAGTGAT
Hairpin Sequence:
5'-CCGG-GCTCTGATCCAGAATAGTGAT-CTCGAG-ATCACTATTCTGGATCAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGATCCAGAATAGTGATCTCGAGATCACTATTCTGGATCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGATCCAGAATAGTGATCTCGAGATCACTATTCTGGATCAGAGC-3'

Other clones with same target sequence:

(none)