Construct: shRNA TRCN0000048575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495882.1-253s1c1
DNA Barcode:
CTCCAGCTAAAGCGCCAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC440046 (440046)
Gene Description:
similar to Itchy homolog E3 ubiquitin protein ligase (Itch) (Atrophin-1-interacting protein 4) (AIP4) (NFE2-associated polypeptide 1) (NAPP1)
Transcript:
RefSeq XM_495882.1 (NON-CURRENT)
Match location:
Position 253 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGCTAAAGCGCCAGCTT
Hairpin Sequence:
5'-CCGG-CTCCAGCTAAAGCGCCAGCTT-CTCGAG-AAGCTGGCGCTTTAGCTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGCTAAAGCGCCAGCTTCTCGAGAAGCTGGCGCTTTAGCTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGCTAAAGCGCCAGCTTCTCGAGAAGCTGGCGCTTTAGCTGGAG-3'

Other clones with same target sequence:

(none)