Construct: shRNA TRCN0000048577
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_495882.1-173s1c1
- DNA Barcode:
- TCAAGAGCTTTGCGGAGCCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LOC440046 (440046)
- Gene Description:
- similar to Itchy homolog E3 ubiquitin protein ligase (Itch) (Atrophin-1-interacting protein 4) (AIP4) (NFE2-associated polypeptide 1) (NAPP1)
- Transcript:
- RefSeq XM_495882.1 (NON-CURRENT)
- Match location:
- Position 173 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000048577)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCAAGAGCTTTGCGGAGCCTA
- Hairpin Sequence:
-
5'-CCGG-TCAAGAGCTTTGCGGAGCCTA-CTCGAG-TAGGCTCCGCAAAGCTCTTGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCAAGAGCTTTGCGGAGCCTACTCGAGTAGGCTCCGCAAAGCTCTTGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCAAGAGCTTTGCGGAGCCTACTCGAGTAGGCTCCGCAAAGCTCTTGA-3'
Other clones with same target sequence:
(none)
