Construct: shRNA TRCN0000048584

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495884.1-437s1c1
DNA Barcode:
GCCCATGAAGCGCCACATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC440054 (440054)
Gene Description:
similar to ARF-GAP, RHO-GAP, ankyrin repeat and plekstrin homology domains-containing protein 3; phosphoinositide binding protein; PtdIns(3,4,5)P3-binding protein
Transcript:
RefSeq XM_495884.1 (NON-CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048584)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATGAAGCGCCACATCTT
Hairpin Sequence:
5'-CCGG-GCCCATGAAGCGCCACATCTT-CTCGAG-AAGATGTGGCGCTTCATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATGAAGCGCCACATCTTCTCGAGAAGATGTGGCGCTTCATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATGAAGCGCCACATCTTCTCGAGAAGATGTGGCGCTTCATGGGC-3'

Other clones with same target sequence:

(none)