Construct: shRNA TRCN0000048637

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_116036.5-250s1c1
DNA Barcode:
CCAGTAGGTATAGATGTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRR3 (200959)
Gene Description:
gamma-aminobutyric acid type A receptor rho3 subunit (gene/pseudogene)
Transcript:
RefSeq XM_116036.5 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048637)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAGGTATAGATGTCCAT
Hairpin Sequence:
5'-CCGG-CCAGTAGGTATAGATGTCCAT-CTCGAG-ATGGACATCTATACCTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAGGTATAGATGTCCATCTCGAGATGGACATCTATACCTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAGGTATAGATGTCCATCTCGAGATGGACATCTATACCTACTGG-3'

Other clones with same target sequence:

(none)