Construct: shRNA TRCN0000048647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014899.2-1600s1c1
DNA Barcode:
GCCGATGTTGTCTTCGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RHOBTB3 (22836)
Gene Description:
Rho related BTB domain containing 3
Transcript:
RefSeq NM_014899.2 (NON-CURRENT)
Match location:
Position 1600 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGATGTTGTCTTCGAAATT
Hairpin Sequence:
5'-CCGG-GCCGATGTTGTCTTCGAAATT-CTCGAG-AATTTCGAAGACAACATCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGATGTTGTCTTCGAAATTCTCGAGAATTTCGAAGACAACATCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGATGTTGTCTTCGAAATTCTCGAGAATTTCGAAGACAACATCGGC-3'

Other clones with same target sequence:

  1. TRCN0000291537