Construct: shRNA TRCN0000048679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_085127.7-1164s1c1
DNA Barcode:
CCAGGAAATTGCCAAGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHG3 (26030)
Gene Description:
pleckstrin homology and RhoGEF domain containing G3
Transcript:
RefSeq XM_085127.7 (NON-CURRENT)
Match location:
Position 1164 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAAATTGCCAAGCATTT
Hairpin Sequence:
5'-CCGG-CCAGGAAATTGCCAAGCATTT-CTCGAG-AAATGCTTGGCAATTTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAAATTGCCAAGCATTTCTCGAGAAATGCTTGGCAATTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAAATTGCCAAGCATTTCTCGAGAAATGCTTGGCAATTTCCTGG-3'

Other clones with same target sequence:

(none)