Construct: shRNA TRCN0000048771

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080658.1-501s1c1
DNA Barcode:
CTTTGTCCTTGACCTGCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACY3 (91703)
Gene Description:
aminoacylase 3
Transcript:
RefSeq NM_080658.1 (NON-CURRENT)
Match location:
Position 501 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048771)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGTCCTTGACCTGCACAA
Hairpin Sequence:
5'-CCGG-CTTTGTCCTTGACCTGCACAA-CTCGAG-TTGTGCAGGTCAAGGACAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGTCCTTGACCTGCACAACTCGAGTTGTGCAGGTCAAGGACAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGTCCTTGACCTGCACAACTCGAGTTGTGCAGGTCAAGGACAAAG-3'

Other clones with same target sequence:

(none)