Construct: shRNA TRCN0000048813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000437.2-773s1c1
DNA Barcode:
CGAGTGTTTACGGGTGTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAFAH2 (5051)
Gene Description:
platelet activating factor acetylhydrolase 2
Transcript:
RefSeq NM_000437.2 (NON-CURRENT)
Match location:
Position 773 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTGTTTACGGGTGTTGAA
Hairpin Sequence:
5'-CCGG-CGAGTGTTTACGGGTGTTGAA-CTCGAG-TTCAACACCCGTAAACACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTGTTTACGGGTGTTGAACTCGAGTTCAACACCCGTAAACACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTGTTTACGGGTGTTGAACTCGAGTTCAACACCCGTAAACACTCG-3'

Other clones with same target sequence:

  1. TRCN0000307218