Construct: shRNA TRCN0000048819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002600.2-525s1c1
DNA Barcode:
GCGCAGAGAGTCATTTCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE4B (5142)
Gene Description:
phosphodiesterase 4B
Transcript:
RefSeq NM_002600.2 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCAGAGAGTCATTTCTCTA
Hairpin Sequence:
5'-CCGG-GCGCAGAGAGTCATTTCTCTA-CTCGAG-TAGAGAAATGACTCTCTGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCAGAGAGTCATTTCTCTACTCGAGTAGAGAAATGACTCTCTGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCAGAGAGTCATTTCTCTACTCGAGTAGAGAAATGACTCTCTGCGC-3'

Other clones with same target sequence:

(none)