Construct: shRNA TRCN0000048891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006821.3-1180s1c1
DNA Barcode:
CGTCAACAGAAATCGCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACOT2 (10965)
Gene Description:
acyl-CoA thioesterase 2
Transcript:
RefSeq NM_006821.3 (NON-CURRENT)
Match location:
Position 1180 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCAACAGAAATCGCATCAA
Hairpin Sequence:
5'-CCGG-CGTCAACAGAAATCGCATCAA-CTCGAG-TTGATGCGATTTCTGTTGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCAACAGAAATCGCATCAACTCGAGTTGATGCGATTTCTGTTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCAACAGAAATCGCATCAACTCGAGTTGATGCGATTTCTGTTGACG-3'

Other clones with same target sequence:

  1. TRCN0000174083
  2. TRCN0000179740