Construct: shRNA TRCN0000048933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017711.2-1084s1c1
DNA Barcode:
CCACGAATGTAGCCTCTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GDPD2 (54857)
Gene Description:
glycerophosphodiester phosphodiesterase domain containing 2
Transcript:
RefSeq NM_017711.2 (NON-CURRENT)
Match location:
Position 1084 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGAATGTAGCCTCTGTAT
Hairpin Sequence:
5'-CCGG-CCACGAATGTAGCCTCTGTAT-CTCGAG-ATACAGAGGCTACATTCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGAATGTAGCCTCTGTATCTCGAGATACAGAGGCTACATTCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGAATGTAGCCTCTGTATCTCGAGATACAGAGGCTACATTCGTGG-3'

Other clones with same target sequence:

(none)