Construct: shRNA TRCN0000048946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018667.2-643s1c1
DNA Barcode:
CCCTTTGCGTTTCTCGGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMPD3 (55512)
Gene Description:
sphingomyelin phosphodiesterase 3
Transcript:
RefSeq NM_018667.2 (NON-CURRENT)
Match location:
Position 643 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTGCGTTTCTCGGCTTT
Hairpin Sequence:
5'-CCGG-CCCTTTGCGTTTCTCGGCTTT-CTCGAG-AAAGCCGAGAAACGCAAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTGCGTTTCTCGGCTTTCTCGAGAAAGCCGAGAAACGCAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTGCGTTTCTCGGCTTTCTCGAGAAAGCCGAGAAACGCAAAGGG-3'

Other clones with same target sequence:

(none)