Construct: shRNA TRCN0000048981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000283.1-519s1c1
DNA Barcode:
GCTCACTGACTACAAGACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE6B (5158)
Gene Description:
phosphodiesterase 6B
Transcript:
RefSeq NM_000283.1 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000048981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACTGACTACAAGACAAA
Hairpin Sequence:
5'-CCGG-GCTCACTGACTACAAGACAAA-CTCGAG-TTTGTCTTGTAGTCAGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACTGACTACAAGACAAACTCGAGTTTGTCTTGTAGTCAGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACTGACTACAAGACAAACTCGAGTTTGTCTTGTAGTCAGTGAGC-3'

Other clones with same target sequence:

(none)