Construct: shRNA TRCN0000049081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005125.1-675s1c1
DNA Barcode:
GCCATCCCTTATCCAAGATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCS (9973)
Gene Description:
copper chaperone for superoxide dismutase
Transcript:
RefSeq NM_005125.1 (NON-CURRENT)
Match location:
Position 675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCCCTTATCCAAGATCA
Hairpin Sequence:
5'-CCGG-GCCATCCCTTATCCAAGATCA-CTCGAG-TGATCTTGGATAAGGGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCCCTTATCCAAGATCACTCGAGTGATCTTGGATAAGGGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCCCTTATCCAAGATCACTCGAGTGATCTTGGATAAGGGATGGC-3'

Other clones with same target sequence:

(none)