Construct: shRNA TRCN0000049085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018290.1-872s1c1
DNA Barcode:
CCTGAGTTTCCAACAGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PGM2 (55276)
Gene Description:
phosphoglucomutase 2
Transcript:
RefSeq NM_018290.1 (NON-CURRENT)
Match location:
Position 872 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGTTTCCAACAGTGAAA
Hairpin Sequence:
5'-CCGG-CCTGAGTTTCCAACAGTGAAA-CTCGAG-TTTCACTGTTGGAAACTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGTTTCCAACAGTGAAACTCGAGTTTCACTGTTGGAAACTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGTTTCCAACAGTGAAACTCGAGTTTCACTGTTGGAAACTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000300517