Construct: shRNA TRCN0000049099

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_372112.2-973s1c1
DNA Barcode:
CCCAGCCAATTCTGCAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PGM5P1 (389753)
Gene Description:
phosphoglucomutase 5 pseudogene 1
Transcript:
RefSeq XM_372112.2 (NON-CURRENT)
Match location:
Position 973 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049099)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCCAATTCTGCAATAAA
Hairpin Sequence:
5'-CCGG-CCCAGCCAATTCTGCAATAAA-CTCGAG-TTTATTGCAGAATTGGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCCAATTCTGCAATAAACTCGAGTTTATTGCAGAATTGGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCCAATTCTGCAATAAACTCGAGTTTATTGCAGAATTGGCTGGG-3'

Other clones with same target sequence:

(none)