Construct: shRNA TRCN0000049172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371409.2-167s1c1
DNA Barcode:
CTGGAGAGAAAGGATTTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC388817 (388817)
Gene Description:
peptidylprolyl isomerase A-like
Transcript:
RefSeq XM_371409.2 (NON-CURRENT)
Match location:
Position 167 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAGAGAAAGGATTTGGTT
Hairpin Sequence:
5'-CCGG-CTGGAGAGAAAGGATTTGGTT-CTCGAG-AACCAAATCCTTTCTCTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAGAGAAAGGATTTGGTTCTCGAGAACCAAATCCTTTCTCTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAGAGAAAGGATTTGGTTCTCGAGAACCAAATCCTTTCTCTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000203719