Construct: shRNA TRCN0000049173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004508.2-540s1c1
DNA Barcode:
CGTGTTGTAGTCATCCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IDI1 (3422)
Gene Description:
isopentenyl-diphosphate delta isomerase 1
Transcript:
RefSeq NM_004508.2 (NON-CURRENT)
Match location:
Position 540 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTTGTAGTCATCCATTAA
Hairpin Sequence:
5'-CCGG-CGTGTTGTAGTCATCCATTAA-CTCGAG-TTAATGGATGACTACAACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTTGTAGTCATCCATTAACTCGAGTTAATGGATGACTACAACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTTGTAGTCATCCATTAACTCGAGTTAATGGATGACTACAACACG-3'

Other clones with same target sequence:

  1. TRCN0000290654