Construct: shRNA TRCN0000049336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004911.3-1441s1c1
DNA Barcode:
GCAAGGTGTCAAACGATGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDIA4 (9601)
Gene Description:
protein disulfide isomerase family A member 4
Transcript:
RefSeq NM_004911.3 (NON-CURRENT)
Match location:
Position 1441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGGTGTCAAACGATGCTA
Hairpin Sequence:
5'-CCGG-GCAAGGTGTCAAACGATGCTA-CTCGAG-TAGCATCGTTTGACACCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGGTGTCAAACGATGCTACTCGAGTAGCATCGTTTGACACCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGGTGTCAAACGATGCTACTCGAGTAGCATCGTTTGACACCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000307107