Construct: shRNA TRCN0000049355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005742.2-385s1c1
DNA Barcode:
GCAGATAAGCATCATTCCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDIA6 (10130)
Gene Description:
protein disulfide isomerase family A member 6
Transcript:
RefSeq NM_005742.2 (NON-CURRENT)
Match location:
Position 385 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATAAGCATCATTCCCTA
Hairpin Sequence:
5'-CCGG-GCAGATAAGCATCATTCCCTA-CTCGAG-TAGGGAATGATGCTTATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATAAGCATCATTCCCTACTCGAGTAGGGAATGATGCTTATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATAAGCATCATTCCCTACTCGAGTAGGGAATGATGCTTATCTGC-3'

Other clones with same target sequence:

(none)