Construct: shRNA TRCN0000049470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003725.2-869s1c1
DNA Barcode:
GCTGTTGAATTGTAGCACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSD17B6 (8630)
Gene Description:
hydroxysteroid 17-beta dehydrogenase 6
Transcript:
RefSeq NM_003725.2 (NON-CURRENT)
Match location:
Position 869 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000049470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGAATTGTAGCACAAA
Hairpin Sequence:
5'-CCGG-GCTGTTGAATTGTAGCACAAA-CTCGAG-TTTGTGCTACAATTCAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGAATTGTAGCACAAACTCGAGTTTGTGCTACAATTCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGAATTGTAGCACAAACTCGAGTTTGTGCTACAATTCAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000292060